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One milliliter of cytosol or actin cytoskeleton (CSK) from resting or thrombin-stimulated platelets (1 × 109/ml platelets) was immunoprecipitated with 5 μl of nonimmune serum or C-terminal 4-phosphatase antibody and immunoblotted with 4-phosphatase antiserum (A) or p85 antiserum (B).
