Table 1.
Proteins dysregulated by perifosine (10 μM for 12 hours) using nanoscale antibody microarray
| Classes, up-regulated proteins | No.* | Down-regulated proteins | No. |
|---|---|---|---|
| Cell-signaling pathways/kinases | |||
| Mitogen-activated protein kinase kinase 4 (JNK-activating kinase 1) | P45985 | Ribosomal protein S6 kinase alpha-1 | Q15418 |
| Mitogen-activated protein kinase 1/ERK2 | P28482 | Inhibitor of nuclear factor kappa-B kinase alpha subunit | O15111 |
| Protein phosphatase 1, regulatory (inhibitor) subunit 2 | P41236 | — | — |
| Phospholipase C, gamma 1 (formerly subtype 148) | P19174 | — | — |
| Protein kinase, cAMP-dependent, regulatory, type I alpha/PRKAR1A | P10644 | — | — |
| Ras-GTPase-activating protein SH3-domain-binding protein | Q13283 | — | — |
| Rho GDP dissociation inhibitor (GDI) beta | P52566 | — | — |
| RAB24, member RAS oncogene family | Q969Q5 | — | — |
| Polo-like kinase (Drosophila) | P53350 | — | — |
| Insulin receptor substrate 1 | P35568 | — | — |
| Active BCR-related gene/ABR | Q12979 | — | — |
| Apoptosis | |||
| Caspase 7, apoptosis-related cysteine protease | P55210 | — | — |
| BCL2 | P10415 | — | — |
| Cell-cycle regulators and inhibition of proliferation | |||
| Transforming growth factor beta 1 induced transcript 1 | O43294 | — | — |
| Cyclin-dependent kinase 7 | P50613 | — | — |
| Leucine zipper putative tumor suppressor 1 | Q9Y250 | — | — |
| CDC37 cell division cycle 37 homolog (S cerevisiae) | Q16543 | — | — |
| Serpin B5 | P36952 | — | — |
| Chromosome/cell division | |||
| Pericentrin 2 (kendrin) | P48725 | MRE11 meiotic recombination 11 homolog A | P49959 |
| Histone deacetylase 3 | O15379 | Microtubule-associated protein 4 | P27816 |
| Thymopoietin | P42166 | DEAH (Asp-Glu-Ala-His) box polypeptide 16 | O60231 |
| Nuclear mitotic apparatus protein 1 | Q14980 | — | — |
| Immunomodulator | |||
| Interleukin 12B | P29459 | Linker for activation of T cells/LAT | O43561 |
| Interleukin 1, beta | P01584 | — | — |
| Semaphorin-4D | Q92854 | — | — |
| Others | |||
| Endoglin (Osler-Rendu-Weber syndrome 1) | P17813 | Junction plakoglobin/Desmoplakin-3 | P14923 |
| Stromal interaction molecule 1 | Q13586 | Adaptor-related protein complex 2, alpha 1 subunit/AP-2A1 | O95782 |
| Potassium voltage-gated channel, subfamily H, member 6 | Q9H252 | Protein kinase, cAMP-dependent, regulatory, type II, beta | P31323 |
| Sorting nexin 1 | Q13596 | — | — |
| Syntaxin binding protein 1 | Q64320 | — | — |
| Neuronal Shc | Q92529 | — | — |
| 5-hydroxytryptamine (serotonin) receptor 2C | P28335 | — | — |
— indicates no entry.
*
Swiss protein accession numbers.