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. 1999 Mar 30;96(7):3646–3651. doi: 10.1073/pnas.96.7.3646

Figure 3.

Figure 3

Calcitonin and amphiregulin mRNA induction by progesterone in the uterus is ERα independent. (A) Uterine poly(A)+ RNA (3 μg/lane) was analyzed by Northern blot as described in Materials and Methods. WT or ERKO mice were ovexed and then treated as described with vehicle (C) or estrogen primed for 1 day and then treated with progesterone for 3 days (P). Duplicate samples were prepared from identically treated animal sets and analyzed in adjacent lanes. The Northern blot was probed with calcitonin cDNA (CALCI) or cyclophilin riboprobe (CYP). The RNA size markers (in kb) are indicated on the upper left. (B) Total uterine RNA (10 μg/lane) was analyzed by Northern blot as described in Materials and Methods. WT or ERKO mice were ovexed and then treated as described with vehicle, progesterone alone (PROG), or estradiol and progesterone (EST + PROG) and sacrificed 4 hr later. Duplicate samples were prepared from identically treated animal sets and analyzed in adjacent lanes. The Northern blot was probed with amphiregulin riboprobe (Top) or cyclophilin riboprobe (Bottom). The position of RNA size markers is indicated in kb.