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Northern blot analysis of MEPI transfection of MDA-MB-435 cells. Total RNAs were isolated from two control pCI-neo-transfected clone and five MEPI-transfected clones. Strong MEPI transcripts were detected in MEPI-positive clones. In contrast, no endogenous MEPI transcripts were detected in control clones. The integrity of the RNAs and loading control were ascertained by visualization of the 18S rRNA bands in stained gel (data not shown).
