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. 1969 Jun;63(2):378–383. doi: 10.1073/pnas.63.2.378

FORMATION AND DETECTION OF RNA-DNA HYBRID MOLECULES IN CYTOLOGICAL PREPARATIONS*

Joseph G Gall 1, Mary Lou Pardue 1
PMCID: PMC223575  PMID: 4895535

Abstract

A technique is described for forming molecular hybrids between RNA in solution and the DNA of intact cytological preparations. Cells in a conventional tissue squash are immobilized under a thin layer of agar. Next they are treated with alkali to denature the DNA and then incubated with tritium-labeled RNA. The hybrids are detected by autoradiography. The technique is illustrated by the hybridization of ribosomal RNA to the amplified ribosomal genes in oocytes of the toad Xenopus. A low level of gene amplification was also detected in premeiotic nuclei (oogonia).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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