Figure 5.

Effect of various cell cycle-modulating genes on the Gadd45-induced G₂/M arrest. Normal primary human fibroblasts were either microinjected alone with GADD45 or coinjected with cyclin B1, cdc25A, cdc25B, cdc25C, or p34^cdc2 (cdc2). Cells were fixed at 24 hr, and Gadd45-positive cells displaying an arrested morphology were scored. At least three independent microinjection experiments were averaged, and data are expressed as mean ± SD. By using Student’s t test, a statistically significant P value (<0.05) was obtained between GADD45 alone and coinjection with cyclin B1 and/or cdc25C.