Table 1.
Bacterial strains and plasmids used in this study.
| Strain/plasmid | Relevant genotype and/or other characteristics | Reference |
| P. fluorescens | ||
| SBW25 | Wild-type | [17] |
| PBR840 | SBW25ΔpvdL or ΔPflu4387 | This study |
| 15F3 | PBR840 with mini-Tn phoA3 disruption of secondary siderophore biosynthesis genes, GmR | S. Matthijs, unpublished |
| TR107.1.1a | SBW25 carrying the pvdS-'lacZ fusion, SmR | This study |
| TR107.2.1a | SBW25 carrying the pvdL-'lacZ fusion, SmR | This study |
| TR107.4.1a | SBW25 carrying the fpvI::'lacZ fusion, SmR | This study |
| TR107.5.1a | SBW25 carrying the fpvR::'lacZ fusion, SmR | This study |
| TR135.1.1a | SBW25 carrying the fpvA::'lacZ fusion, SmR | This study |
| TR137.2.1 | TR107.2.1 with pTr130.1, GmR | This study |
| TR137.3.1 | TR107.2.1 with pTr130.2, GmR | This study |
| TR137.4.1 | TR107.2.1 with pTr130.3, GmR | This study |
| TR156.1.1 | TR107.2.1 with pBroadgate-D, GmR | This study |
| TR138.2.1 | TR135.1.1 with pTr130.1, GmR | This study |
| TR138.3.1 | TR135.1.1 with pTr130.2, GmR | This study |
| TR138.4.1 | TR135.1.1 with pTr130.3, GmR | This study |
| TR156.3.1 | TR135.1.1 with pBroadgate-D | This study |
| TR168.1.1 | TR107.5.1 with pTr130.1, GmR | This study |
| TR168.2.1 | TR107.5.1 with pTr130.2, GmR | This study |
| TR168.3.1 | TR107.5.1 with pTr130.3, GmR | This study |
| TR168.4.1 | TR107.5.1 with pBroadgate-D | This study |
| Plasmids | ||
| pUIC3 | Integration vector with 'lacZ, TcR | [20] |
| pHP45Ω | Source of the omega cassette, SmR | [51] |
| pIVET-Sm | pUIC3 with tet gene replaced by Smr/Spr Ω fragment from pHP45Ω, SmR | This study |
| pUIC3–40 | pUIC3 containing pvdL deletion fragment | This study |
| pCR2.1 | PCR product cloning vector, KmR | Invitrogen |
| pRK2013 | helper plasmid, KmR, Tra+ | [52] |
| pTR100.1.1 | pIVET-Sm carrying the pvdS-'lacZ fusion | This study |
| pTR100.2.1 | pIVET-Sm carrying the pvdL-'lacZ fusion | This study |
| pTR100.4.1 | pIVET-Sm carrying the fpvI-'lacZ fusion | This study |
| pTR100.5.1 | pIVET-Sm carrying the fpvR-'lacZ fusion | This study |
| pTR128.1.1 | pIVET-Sm carrying the fpvA-'lacZ fusion | This study |
| pBroadgate | Gateway expression vector with Plac promoter, GmR | Thwaites and Mansfield |
| pBroadgate-D | pBroadgate with ccdB gene replaced with the linker fragment, GmR; | [53] |
| pTR130.1 | pBroadgate::pvdS, GmR | This study |
| pTR130.2 | pBroadgate::fpvI, GmR | This study |
| pTR130.3 | pBroadgate::fpvR, GmR | This study |
a The 'lacZ fusion reporter was integrated into the PVD locus by insertion-duplication thus the gene function was not affected.