Table 3.
Oligonucleotide primers used in this study.
| Primer | Sequence (5'-3')a | Application |
| CM-030 | GGGAGATCTAGGTCCACATTGGATTGCAGG | pvdS promoter |
| CM-038 | CCGCTCGAGGGTGAAGCGCCGTGAATAACCACA | pvdS promoter |
| CM-036 | GGAAGATCTCCTGTGGATACTTGTTCCGTCA | pvdL promoter |
| CM-037 | CCGCTCGAGACCAAAGAACGCCGCGACGTAATC | pvdL promoter |
| CM-050 | GGAAGATCTCTGCTGGGCGTTGAGGGTCTGG | fpvI promoter |
| CM-051 | CCGCTCGAGCGGGAAATGCACAGGCGTTCGGCA | fpvI promoter |
| CM-052 | GGAAGATCTCGTCTGCAGCGTCTGGTCCTGGAG | fpvR promoter |
| CM-053 | CCGCTCGAGGTCCTTGTAGTTGCTGTAGACCAG | fpvR promoter |
| CM-116 | GGGAGATCTGAAGTAGGAACGTGTCACTAGTGG | fpvA promoter |
| CM-117 | GGGCTCGAGGTCGAAGTGCCTGCCAGCAAG | fpvA promoter |
| CM-110 | CACCGTGTAGGAGGCCTCAGCAAGG | pvdS ORF |
| CM-111 | ACATAAGGCGCACATTTCATCTGC | pvdS ORF |
| CM-112 | CACCACTCAGGGACCTAATGCCGC | fpvI ORF |
| CM-113 | CATGTGTTGATAGGAAACGAGGAC | fpvI ORF |
| CM-114 | CACCCGTGAAGTTAGAATGTGGG | fpvR ORF |
| CM-115 | TGGGCGGTTCAGGGCATG | fpvR ORF |
| pvdL1 | GAAGATCTTCGAACTTCCCCAAACGCT | pvdL deletion |
| pvdL2 | CAGCATGCGGATCCGTTGACGGAACCCGCAGCGCGGGGATGCC | pvdL deletion |
| pvdL3 | TCCGTCAACGGATCCGCATGCTGTGGCGCACCAATGGCTGCT | pvdL deletion |
| pvdL4 | GAAGATCTCCAACTCCGCCATCAAATCA | pvdL deletion |
a Underlined sequences denote BglII or XhoI restriction sites to facilitate cloning; sequences in bold type are tag for directional topoisomerase-mediated cloning, and sequences in italics denote random complementary sequences to facilitate SOE-PCR [50].