Table 3.

Parameters of the geNorm software for ACTB, SDHA, and HPRT. The parameters were determined from quantities (R₀ values) of the three genes in the RT-PCR template dilutions. M – measure of the gene expression stability, V2/V3 – pairwise variations determining the optimal number of reference genes. Indicated methods for real-time PCR data analysis were used for the transformation of fluorescence data to the R₀ values. Designation of the methods is the same as in Table 2.

Parameter/Method	St cur	Comp	SCF	DART ind E	DART av E	Liu&S ind E	Liu&S av E	LinReg ind E	LinReg-Ct av E
M for ACTB	0.159	0.161	0.315	0.598	0.200	0.544	0.213	1.772	0.276
M for SDHA	0.187	0.191	0.293	0.578	0.183	0.538	0.326	1.549	0.246
M for HPRT	0.181	0.165	0.324	0.631	0.226	0.626	0.228	1.349	0.338
V2/V3	0.057	0.060	0.097	0.190	0.071	0.195	0.107	0.564	0.109