Table 4.

Intra-assay variability. Coefficients of variation calculated from R₀ values of 15 replicate PCR reactions. Indicated methods for real-time PCR data analysis were used for the transformation of fluorescence data to the R₀ values. Designation of the methods is the same as in Table 2.

Gene/Method	St cur	Comp	SCF	DART ind E	DART av E	Liu&S ind E	Liu&S av E	LinReg ind E	LinReg-Ct av E
IL-1β	5.95	5.95	17.1	60.6	7.63	25.03	7.33	49.08	5.71
IL-6	9.94	9.94	22.3	83.6	10.2	43.7	9.28	53.5	9.73