Table 5.
Inter-assay variability. Coefficients of variation calculated from R0 values of 8 PCR reactions. Indicated methods for real-time PCR data analysis were used for the transformation of fluorescence data to the R0 values. Designation of the methods is the same as in Table 2.
Gene/Method | St cur | Comp | SCF | DART ind E | DART av E | Liu&S ind E | Liu&S av E | LinReg ind E | LinReg-Ct av E |
IL-1β | 12.4 | 12.4 | 15.3 | 79.7 | 18.3 | 30.3 | 17.7 | 68.8 | 11.2 |
IL-6 | 18.6 | 17.1 | 20.5 | 65.5 | 17.7 | 40.2 | 17.5 | 53.8 | 16.2 |