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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1969 Jun;63(2):520–527. doi: 10.1073/pnas.63.2.520

TWO RAPIDLY LABELED RNA SPECIES IN THE POLYSOMES OF ANTIBODY-PRODUCING LYMPHOID TISSUE

Ernst Kuechler 1, Alexander Rich 1
PMCID: PMC223594  PMID: 4979327

Abstract

Popliteal lymph nodes of rabbits stimulated to produce antibodies were pulse labeled in vivo by direct injection of 3H-uridine. RNA was then extracted from isolated polysomes and single ribosomes. Fractionation of this RNA by polyacrylamide gel electrophoresis revealed the synthesis of two discrete peaks of labeled RNA migrating in the region between 18S ribosomal and 4S transfer RNA. These peaks were found in the RNA extracted from polysomes but were absent from single ribosomes. When the polysomes were disrupted by EDTA treatment the RNA species no longer appeared as rapidly sedimenting material. These two RNA's have molecular weights near 2.2 × 105 and 3.7 × 105 daltons. The chemical and biological properties of these species as well as the molecular weights are consistent with those expected for monocistronic messenger RNA coding for the antibody L and H chains, respectively.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Becker M. J., Rich A. Polyribosomes of tissues producing antibodies. Nature. 1966 Oct 8;212(5058):142–146. doi: 10.1038/212142a0. [DOI] [PubMed] [Google Scholar]
  2. Bishop D. H., Claybrook J. R., Spiegelman S. Electrophoretic separation of viral nucleic acids on polyacrylamide gels. J Mol Biol. 1967 Jun 28;26(3):373–387. doi: 10.1016/0022-2836(67)90310-5. [DOI] [PubMed] [Google Scholar]
  3. Borun T. W., Scharff M. D., Robbins E. Rapidly labeled, polyribosome-associated RNA having the properties of histone messenger. Proc Natl Acad Sci U S A. 1967 Nov;58(5):1977–1983. doi: 10.1073/pnas.58.5.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Choules G. L., Zimm B. H. An acrylamide gel soluble in scintillation fluids: its application to electrophoresis at neutral and low pH. Anal Biochem. 1965 Nov;13(2):336–344. doi: 10.1016/0003-2697(65)90202-2. [DOI] [PubMed] [Google Scholar]
  5. Loening U. E., Ingle J. Diversity of RNA components in green plant tissues. Nature. 1967 Jul 22;215(5099):363–367. doi: 10.1038/215363a0. [DOI] [PubMed] [Google Scholar]
  6. Mach B., Vassalli P. Biosynthesis of RNA in antibody-producing tissues. Proc Natl Acad Sci U S A. 1965 Sep;54(3):975–982. doi: 10.1073/pnas.54.3.975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Penman S. RNA metabolism in the HeLa cell nucleus. J Mol Biol. 1966 May;17(1):117–130. doi: 10.1016/s0022-2836(66)80098-0. [DOI] [PubMed] [Google Scholar]
  8. Penman S., Vesco C., Penman M. Localization and kinetics of formation of nuclear heterodisperse RNA, cytoplasmic heterodisperse RNA and polyribosome-associated messenger RNA in HeLa cells. J Mol Biol. 1968 May 28;34(1):49–60. doi: 10.1016/0022-2836(68)90234-9. [DOI] [PubMed] [Google Scholar]
  9. Ralph P., Becker M., Rich A. Immunoglobulin synthesis in a cell-free system. Cold Spring Harb Symp Quant Biol. 1967;32:277–282. doi: 10.1101/sqb.1967.032.01.039. [DOI] [PubMed] [Google Scholar]
  10. Schubert D. Immunoglobulin assembly in a mouse myeloma. Proc Natl Acad Sci U S A. 1968 Jun;60(2):683–690. doi: 10.1073/pnas.60.2.683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Shapiro A. L., Scharff M. D., Maizel J. V., Jr, Uhr J. W. Polyribosomal synthesis and assembly of the H and L chains of gamma globulin. Proc Natl Acad Sci U S A. 1966 Jul;56(1):216–221. doi: 10.1073/pnas.56.1.216. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Shapiro A. L., Scharff M. D., Maizel J. V., Uhr J. W. Synthesis of excess light chains of gamma globulin by rabbit lymph node cells. Nature. 1966 Jul 16;211(5046):243–245. doi: 10.1038/211243a0. [DOI] [PubMed] [Google Scholar]
  13. Weinberg R. A., Loening U., Willems M., Penman S. Acrylamide gel electrophoresis of HeLa cell nucleolar RNA. Proc Natl Acad Sci U S A. 1967 Sep;58(3):1088–1095. doi: 10.1073/pnas.58.3.1088. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Williamson A. R., Askonas B. A. Biosynthesis of immunoglobulins: the separate classes of polyribosomes synthesizing heavy and light chains. J Mol Biol. 1967 Jan 28;23(2):201–216. doi: 10.1016/s0022-2836(67)80027-5. [DOI] [PubMed] [Google Scholar]

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