Table 1.
Proliferative potential of cells transduced with hTERT virus
| Cells | Exp. | Virus | No. plates | Cumulative dilution |
|---|---|---|---|---|
| 3A | 1 | Vector | 15 | 7–11 |
| hTERT-HA | 36 | 8–12* | ||
| 2 | Vector | 2 | 16 | |
| hTERT | 4 | >90 | ||
| 3 | Vector | 4 | 18 | |
| D868A | 4 | 18–21 | ||
| hTERT | 4 | >60 | ||
| PLR | 1 | Vector | 6 | 13–15 |
| hTERT-HA | 8 | 13–17 | ||
| hTERT | 8 | >70 | ||
| 2 | Vector | 4 | 12 | |
| D868A | 4 | 12–14 | ||
| hTERT | 4 | >50 | ||
| 3C | Vector | 4 | 8 | |
| D868A | 4 | 8 | ||
| hTERT | 4 | >50 |
The cells were infected with recombinant viruses and selected as pools with puromycin. The cells were passaged when they became confluent, and the numbers in the last column indicate total number of 2-fold dilutions. All hTERT cells were still proliferating when the manuscript was submitted.
*
Colonies appeared in some plates 2–4 months after the cells entered crisis.