Table 2.
Expression of hTERT suppresses the formation of dicentric chromosomes
| No. dicentric chromosomes per cell | PLR cells
|
3A cells
|
||
|---|---|---|---|---|
| hTERT | Vector | hTERT | Vector | |
| 0 | 51/88% | 21/41% | 19/54% | 0/0% |
| 1 | 7/12% | 20/39% | 10/29% | 7/58% |
| 2 | 0/0% | 4/8% | 6/27% | 3/25% |
| ≥3 | 0/0% | 5/10% | 0/0% | 2/17% |
| Total no. metaphase | 58 | 51 | 35 | 12 |
| No. dicentric chromosomes per cell | 0.1 | 0.9 | 0.6 | 1.6 |
Exponentially growing 3A and PLR cells were arrested with Colcemid, and metaphase spreads were prepared on glass slides. The slides then were stained with Giemsa staining solution and examined. The number of dicentric chromosomes in each metaphase spread was scored.