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Localization of VirD2 and VirE2 proteins in HeLa nuclei. Cy3.5-labeled proteins (red fluorescence) were incubated with digitonin-permeabilized HeLa cells for 20 min at room temperature in the dark. The nuclei were stained with SYTO 13 dye (green fluorescence). (A) Localization of VirD2 protein. (B) Localization of VirE2 protein. (C) Localization of BSA.
