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Formation of T-DNA complex in vitro. (A) Scheme of the cleavage assay. (B) Cleavage of 1-kb ssDNA by VirD2wt (lane 2) or mutant VirD2ΔNLS protein (lane 3), control without protein (lane 1). The efficiency of the cutting reaction by VirD2 proteins was tested on rhodamine-labeled 1-kb ssDNA. (C) ssDNA binding activity of VirE2. Increasing amounts of VirE2 protein were added to ssDNA in amounts indicated on the picture.
