Figure 3.

Import of 1-kb ssDNA into HeLa nuclei requires both VirD2 and VirE2 proteins. Different ssDNA–protein complexes containing rhodamine-labeled ssDNA (red fluorescence) were incubated with permeabilized HeLa cells for 20 min at room temperature in the dark. (A) ssDNA alone. (B) VirD2–ssDNA–VirE2 complex. The position of the nuclei is indicated by staining with SYTO 13 dye (green fluorescence). The fluorescently labeled component is indicated by ∗.