Table 1.
Nuclear import of ssDNA depends on VirD2 and VirE2
| Import substrate | Import |
|---|---|
| BSA | − |
| BSA-NLS | + |
| VirE2 | + |
| VirD2 | + |
| ssDNA | − |
| ssDNA–VirD2 | − |
| ssDNA–VirE2 | − |
| ssDNA–VirD2–VirE2 | + |
| ssDNA–VirD2ΔNLS–VirE2 | − |
Import of substrates into mammalian nuclei was tested in vitro using permeabilized HeLa cells and HeLa-derived import factors. Each experiment was repeated at least three times, and for each sample at least 100 nuclei were scored. +, 99–100% of nuclei showed red fluorescence; −, 0–1% of nuclei showed red fluorescence.