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. 1969 Apr;62(4):1116–1120. doi: 10.1073/pnas.62.4.1116

ON THE MECHANISM OF PEPSIN ACTION*

T Ralph Hollands 1,2, Joseph S Fruton 1,2
PMCID: PMC223622  PMID: 4894688

Abstract

The problem of the mechanism of pepsin action is considered in relation to recent data on the kinetics and specificity of the enzyme, as well as the finding, reported here, that pepsin exhibits a deuterium isotope effect in the cleavage of a peptide bond.

The kinetic parameters for the hydrolysis of the Phe(NO2)-Phe bond of Gly-Gly-Gly-Phe(NO2)-Phe-OMe by pepsin have been determined in H2O and in D2O. The finding of a significant deuterium isotope effect (kH2O/kD2O = ca. 2) supports the hypothesis that the catalytic mechanism of pepsin involves the participation, in the rate-limiting step, of a proton donor (probably an enzymic carboxyl group) in addition to an enzymic carboxylate group acting as a nucleophile.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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