Figure 1.
Alpha-SNAP involvement in apical membrane trafficking. (A) Dose-dependent decrease of the TGN to the apical and to the basolateral plasma membrane transport by antibodies against alpha-SNAP. The transport was monitored in SLO-permeabilized, filter-grown MDCK cells that were infected with either influenza virus or VSV. HA and VSV G were used as apical and basolateral markers, respectively. Note that the basolateral transport is more affected than the apical transport by the addition of the 3E2 antibody. (B) Influence of the His6-tagged wild-type and dominant inhibitory mutant (L294A) recombinant alpha-SNAP proteins on apical delivery.