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. 1999 Mar 30;96(7):3739–3744. doi: 10.1073/pnas.96.7.3739

Figure 2.

Figure 2

Effects of Scar constructs, Arp2/3 complex, and profilin on the time course of actin polymerization. (A) No profilin. (B) Acanthamoeba profilin-II at 7.5 μM. (A and B) Conditions: 2.8 μM Acanthamoeba actin monomers, 50 mM KCl, 1 mM MgCl2, 1 mM EGTA, 0.5 mM DTT, 0.1 mM CaCl2, 0.2 mM ATP, 3 mm NaN3, and 10 mM imidazole (pH 7.0) at 22°C. The polymerization of actin was monitored by pyrene fluorescence. (●) No additions to actin; (○) 100 nM Arp2/3 complex; (▴) 50 nM full-length Scar/100 nM Arp2/3 complex; (▵) 50 nM Scar-PWA/100 nM Arp2/3 complex; (▪) 50 nM Scar-WA/100 nM Arp2/3 complex; (▫) 50 nM Scar-W/100 nM Arp2/3 complex; (▾) 50 nM Scar-A/100 nM Arp2/3 complex; (▿) 50 nM Scar-PWA (A) or 50 nM full length Scar (B). Without Arp2/3 complex, these concentrations of Scar constructs have no effect on actin polymerization in either the presence or absence of profilin-I or profilin-II.