Figure 2.

Expression of HuCds1 mRNA and protein. (a) Northern blots containing polyadenylated RNA (2 μg) from the indicated tissues (Left) and cell lines (Right) were probed with HuCds1 cDNA. (b) Cell extracts from different human tissues (80 μg each lane) were electrophoresed on an SDS gel (4–20%), transferred to a nitrocellulose filter, and immunoblotted with affinity-purified rabbit polyclonal antibody raised against HuCds1. (c) Plasmids expressing GFP fused to the carboxyl terminus of HuCds1 (Upper) or GFP alone (Lower) were transiently transfected into HeLa cells by the calcium phosphate method and 48 hours later, the GFP signal was detected and photographed (×40).