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Loss of function of Sec24p mutated in the putative zinc finger. The temperature-sensitive mutants sec24–11 (A) and sec23–1 (B) were transformed with the multicopy 2 μ-based vector pRS326 (2 μ) or the single copy, centromere-containing vector pRS316 (CEN) carrying the genes indicated. Transformants were grown on agar plates in selective media for 3 days at 35°C (nonpermissive temperature for both mutants). Only wild-type genes could rescue the mutants.
