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. 1999 Mar 30;96(7):3763–3768. doi: 10.1073/pnas.96.7.3763

Figure 2.

Figure 2

Cells isolated from Mkk3 (−/−) mice do not express the MKK3 protein kinase. Wild-type (+/+) and Mkk3 (−/−) MEF were prepared, and extracts (30 μg) were examined by protein immunoblot analysis by using antibodies to MKK3, MKK4, MKK6, JNK, and p38 MAP kinase. Open arrows indicate the migration of the protein kinases. Solid arrows indicate the migration of molecular mass standards (kDa).