Figure 5.

Effect of Mkk3 gene disruption on IL-1 and IL-6 gene expression. Wild-type (+/+) and Mkk3 (−/−) MEF were untreated or treated with TNFα (10 ng/ml) for 6 hr. The amount of IL-1α (A), IL-1β (B), IL-6 (C), TNFα (D), IL-1 receptor antagonist (IL-1RA) (E), and glyceraldehyde-3-phosphate dehydrogenase (F) mRNA was measured by ribonuclease protection assay. The protected RNA was detected by autoradiography (Inset) after denaturing PAGE and was quantitated by PhosphorImager analysis (relative PhosphorImager units). For clarity, the time employed for autoradiography was different for each type of mRNA. Similar data were obtained in three separate experiments.