Figure 5.

Shp2 is required for cell migration. (A) Boyden chamber migration assays with wt (a and c) or Shp2 mutant (b and d) cells challenged with PDGF (a and b) or aFGF (c and d) as chemoattractive agents. Photos were taken with brightfield optics with the ×20 objective. Hematoxylin stains cell nuclei purple (arrow, a); the pores on the membrane through which the cells migrate are clearly visible (arrowhead, d). Similar results were obtained in three separate experiments by using two different Shp2 mutant cell lines. (B) Boyden chamber migration assays comparing Shp2 mutant cells infected with a control retrovirus (white bars) or a retrovirus containing the Shp2 gene (blue bars). Reexpressing Shp2 within the mutant fibroblast cells rescues their ability to respond to aFGF as a chemotactic agent. Shown is the average number of cells from five random fields of view within one membrane. Similar results were obtained in three separate experiments using two different rescued cell lines.