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. 1999 Mar 30;96(7):3813–3818. doi: 10.1073/pnas.96.7.3813

Figure 1.

Figure 1

Exon 3-containing RNA is not subject to mRNA surveillance. RNA (15 μg/lane) from wild-type and smg-2 (r863) strains was analyzed by Northern blot for alternatively spliced forms of uaf-1 mRNA. Lanes 1–3 contain RNA from L1 and L3 larvae and adult-stage worms of a wild-type strain. Lanes 4–6 contain RNA from the same stages of smg-2 (r863) worms. uaf-1 RNAs were detected with a random-primed uaf-1 cDNA probe. The 1.9-kb band represents the RNA that retains exon 3; the 1.7-kb RNA is uaf-1 mRNA. The faint 1.8-kb band in lane 4 is unidentified.