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. 1999 Mar 30;96(7):3813–3818. doi: 10.1073/pnas.96.7.3813

Figure 2.

Figure 2

Exon 3 prevents expression of a vit/gfp reporter. Exon 3 was inserted in both orientations into the 3′ UTR of the vit/gfp construct shown in A to create gfpex3 and gfpex3R. Probes used for RNase protection and in situ hybridization are shown. GFP expression in adult hermaphrodite (smg-2) worms: BL3345, gfpex3R: high-level GFP (B); BL3340, gfpex3 (C). (D) One worm each of BL3340 and BL3345 for comparison: gfpex3 (Left) has yellow color because of autofluorescent gut granules; gfpex3R (Right) has abundant GFP expression, identical to vit/gfp (not shown). A–D are at approximately the same magnification.