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. 1999 Mar 30;96(7):3813–3818. doi: 10.1073/pnas.96.7.3813

Figure 4.

Figure 4

Exon 3 causes nuclear retention of vit/gfp mRNA. Micrographs of dissected worms hybridized in situ with digoxigenin-labeled gfp probes. Light blue/whitish color is 4′,6-diamidino-2-phenylindole (DAPI) staining of nuclei; dark color is the product of alkaline-phosphatase coupled to the antidigoxigenin antibody used for detection of in situ hybridization. A–M show hybridization to the antisense probe unless otherwise indicated. Controls: N2 (wild type nontransgenic) (A); BL3340 (gfpex3), sense probe (M). Experimentals (all smg-2): gfpex3R–BL3345 (B–F); gfpex3–BL3340 (G–L). Bars = 100 μ. H and I are at the same magnification and share a scale bar.