Table 1.

GFP expression in transgenic strains

Strain	smg-2			smg+
	++	+	−	++	+	−	PCR*
gfpex3
BL3314	0	7	13	0	0	20
	0	15	11
BL3315	0	17	3	0	0	20
	0	6	21
BL3316	0	11	9	0	0	20
	0	15	10
BL3307				0	0	50	Y
BL3310				0	0	40	Y
BL3312				0	0	32	Y
BL3308				0	0	50	Y
BL3313				0	0	167	Y
BL3309				0	0	77	Y
gfpex3R
BL3324	16	4	0	0	10	10
BL3325	15	2	2	0	5	15
BL3326	19	1	0	12	8
BL3306				0	0	25†
BL3320				0	0	9
BL3321				0	40	34
BL3322				0	25	27

Zeiss Axioskop was used to score worms for fluorescence as (++) strong, (+) weak, or (−) no intestinal GFP expression. The number of worms in each category is listed for each strain. Smg+ transgenic strains arose from germ-line injection into N2 (BL3306–3310, BL3312–3313, BL3320–3322) or by outcrossing smg-2 strains BL3314–3316 and BL3324–3326 with wild type (N2). 

^*Six nonexpressing gfpex3 strains were checked by PCR to verify that they contained the inejcted plasmid DNA (Y). 

^†Some GFP expression was seen in this strain although the frequency was not recorded.