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. Author manuscript; available in PMC: 2009 Jan 1.
Published in final edited form as: Brain Res Rev. 2007 Jul 27;57(1):86–93. doi: 10.1016/j.brainresrev.2007.06.018

Fig. 1. Imaging structure and function in vivo with genetic tools.

Fig. 1

A. Confocal image showing detailed morphology from an intact living fish of an ascending interneuron labeled with GFP under control of the promoter for the engrailed-1 gene, which is expressed in ascending inhibitory interneurons in zebrafish. The view is a lateral view with the head to the left. B. Two ascending interneurons expressing the genetic calcium indicator cameleon 2.1 under control of the engrailed-1 promoter. The calcium response of the neuron marked by the asterisk is shown in C during a swimming episode that begins at the break in the plot. The neurons are active duringswimming, as indicated by the fluorescence ratio increase.