FIGURE 2.

Hepatic Dnmt1 protein levels in rats fed the FMD diet or a methyl-adequate diet for different times. The nuclear extracts were separated by SDS-polyacrylamide (7.5% acrylamide) gel electrophoresis and subjected to immunoblot analysis with respective antibodies. The blot was reprobed with β-tubulin antibody. HRP-conjugated anti-rabbit was used as a secondary antibody and the signal was developed using ECL reagent; the scanned X-ray film was quantified by Kodak Imaging software.