Figure 2.

(A) High copy of DRC1 rescues the growth defect of dpb11–1. dpb11–1 containing either pRS326 (2 μ URA3) or pHW21(2 μ URA3 DRC1) were struck out on SC-Ura plates and incubated at 24°C and 34°C. (B) Conservation between Drc1 and a related protein from S. pombe. Identities are shown as black boxes and similarities as shaded boxes. (C) High-copy DRC1 suppresses the lethality of pol2 mutants. TC102–2D (POL2), TC102–2–11 (pol2–11), TC102–12 (pol2–12) (19), YHA300 (POL2), and YHA301 (pol2–18) (20) strains were transformed with either pRS326 (−) or pHW21(DRC1) (+). Cultures of those transformants were grown in SC-Ura medium at 24°, then serial dilutions of 10⁵, 10⁴, 10³, 10², and 10 cells were spotted onto SC-Ura plates and incubated at the indicated temperatures.