Figure 5.

DPB11 and DRC1 show reciprocal suppression and their proteins physically associate. (A) The Ts phenotype of drc1–1 is suppressed by the overproduction of DPB11. Y799 (drc1–1) cells harboring either an empty vector, pRS326, or DPB11 on a 2-μ plasmid, YEp195DPB11, were struck upon SC-Ura plates and incubated at 24°C, 30°C, and 37°C respectively. (B) Physical interaction between Dpb11 and Drc1 in vitro. GST or GST-Dpb11 bound to glutathione beads purified from baculovirus-infected insect cells were incubated with ³⁵S-methionine-labeled Drc1 made by in vitro translation. Proteins bound to the beads after several washes were resolved by SDS/PAGE (10%) and visualized by autoradiography.