TABLE 1.

Reactivities of MAbs against Aspergillus by IFA, ELISA, and IHC

MAb	Isotype	Reactivity by IFAa										A. fumigatus ELISA result (OD450)a		Reactivity by IHCa,b
		A. fumigatus		A. flavus		A. niger		A. terreus		A. nidulans				A	B	C
		Conidia	Hyphae	Conidia	Hyphae	Conidia	Hyphae	Conidia	Hyphae	Conidia	Hyphae	EAs	MAs
MA7	IgG3	++++	++++	+++	+++	−	+++	−	−	+++	+++	2.038	2.304	+++	++	+++
EA4	IgM	++	+++	+	++++	+	++	−	−	+	++	1.576	2.058	++	+++	++
EA5	IgM	++	+++	+	++++	+	+	−	−	++	+	1.777	2.284	++	++	+++
EA8	IgM	++++	++++	++++	++++	+++	++	−	−	++	+	1.945	2.333	++	+	+++
MA4	IgM	++++	++++	++++	++++	++	+	−	−	++	++	2.233	2.55	++	++	+++
EA6	IgM	++++	++++	++	++++	++	++	−	−	+++	+	1.938	2.460	++	++	++
EA2	IgG1	−	++	−	−	−	−	−	+	−	−	2.261	2.861	+++	++	+
EA3	IgG1	−	++	−	+	−	−	−	+	−	−	2.350	2.950	+	+	+
MA1	IgG1	−	++	−	−	−	−	−	−	−	+	1.620	2.358	+	−	+
EA1	IgG1	+	++	−	−	−	+	−	−	−	−	1.538	2.407	+	+++	+
Con1	IgM	++	++	++	+++	+	+	++	+++	+++	+++	1.731	2.548	+	−	−
Con2	IgG3	+++	++	++	++	++	+	+++	++	++	++	1.676	2.558	+	−	++
EA7	IgM	+++	+++	++	++++	++	++	++	++++	−	+++	2.343	2.302	+	+	−
MA2	IgG1	++	++++	++	+++	++	++++	+++	++++	+	++	2.432	1.957	++	++	−
MA3	IgG1	+	+++	+	+++	++	+++	+++	+++	−	−	1.893	2.668	++	+++	+
MA5	IgG1	−	++	−	+	−	+	−	+	−	−	1.841	2.513	+	+	+
MA6	IgG1	++	++++	++	++	+	+++	+++	++++	+	++++	2.344	2.344	++	+	−

^aThe reactivity of each MAb was determined by IFA to the mycelia and conidia of Aspergillus species, by indirect ELISA to the MAs and EAs of A. fumigatus, and by immunohistochemical assay (IHC) to formalin-fixed paraffin section of tissues. −, no reactivity; +, weak reactivity; ++, intermediate reactivity; +++, strong reactivity; ++++, very strong reactivity.

^bA, kidney tissue from an experimental rabbit model of IA; B, lung tissue from a patient with IA; C, lung tissue from a patient with aspergilloma.