TABLE 1.

Cp values of SYBR green I amplification products from serum samples spiked with cells of Brucella abortus B-19

No. of cells spikeda	Amt of DNAb (fg)	Cp of amplification products extracted from serum samples with the following vol (ml) by boilingc:
		0.2	0.4	0.6	0.8	1
2 × 107	108	21.38 ± 0.05	21.37 ± 0.03	22.11 ± 0.06	23.47 ± 0.04	24.31 ± 0.04
2 × 106	107	26.13 ± 0.03	26.29 ± 0.04	28.21 ± 0.03
2 × 105	106	28.50 ± 0.06	28.31 ± 0.04	29.15 ± 0.02
2 × 104	105	30.37 ± 0.02	30.19 ± 0.06	31.24 ± 0.05
2 × 103	104	31.71 ± 0.03	31.92 ± 0.03	32.98 ± 0.02
2 × 102	103	34.09 ± 0.04	33.89 ± 0.04	35.44 ± 0.04
20	102	37.79 ± 0.08	38.43 ± 0.05	39.88 ± 0.06
2	10	39.77 ± 0.16	39.92 ± 0.09	30.35 ± 0.14

^aNumber of cells added in different serum volumes assayed.

^bAmount of DNA in the different serum volumes assayed.

^cThe results are the means ± standard deviations of three experiments.