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DTH responses to LPS-free peptide NC-1130-immunized mice. Ear swelling was measured 24 h after injecting 15 μg peptide in the right ear pinna and PBS in the left ear as a control. C57BL/6 mice were immunized with 0, 5, 20, or 100 μg peptide nasally three times prior to testing for DTH responses. Indicated are the observed mean ear swelling values plus 1 standard deviation for five mice per group. The ear swelling units are expressed in inches (104).
