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. 2007 Dec 12;15(2):267–276. doi: 10.1128/CVI.00284-07

FIG. 3.

FIG. 3.

LC-MS analysis of the suspect contaminated Gag 114 peptide from manufacturer A (A to C) and the in-house-resynthesized Gag 114 peptide (D to F). (A and D) UV traces of HPLC. High peptide purity is evident. The selected ion mode with mass filter adjusted to the calculated theoretical mass of the Gag 114 peptide (1,559.80 [M + H]+) is shown for each peptide (B and E). The desired product is clearly present in both profiles. The selected ion mode with mass filter adjusted to the calculated theoretical mass of the HCMV-HCMV-NV9 peptide (944.18 [M + H]+) is shown (C and F). A signal corresponding the exact theoretical molecular weight (MW) of HCMV-NV9 is present in the outsourced Gag 114 peptide. Note the different scales on the y axes.