FIG. 5.

The three stimulatory peptides, Env 97, Env 137, and Pol 31, mediate their effect in an HLA-A0201-dependent manner. Cryopreserved PBMC from three HIV-1-seronegative subjects were tested for their abilities to respond to Env 97, Env 137, and Pol 31 peptides alone or pulsed onto autologous and partially HLA-matched BLCL in a CFC assay for IFN-γ production. The percentage of IFN-γ-positive cells within the gated CD3⁺ CD8⁺ T-cell population is shown. The HLA type of each subject and cocultured BLCL is shown below the bar graphs. The common allele shared by the responding subject and stimulating peptide-pulsed BLCL is shown.