FIG. 2.

OVA-specific IgG, IgG1, and IgG2a antibody responses in BALB/c mice immunized s.c. with OVA (10 μg) in saline (200 pl) alone or with OVA (10 μg) in saline (200 pl) containing alum (200 μg), Rg1 (50 μg), or alum (200 μg) plus Rg1 (50 μg) on days 1 and 21. Serum was collected 2 weeks after the boosting. Serum OVA-specific IgG, IgG1, and IgG2a antibody levels were measured by an indirect ELISA. The values are presented as means ± standard deviations (n = 5). Values with different letters showed significant difference (P < 0.05).