FIG. 4.

IL-5 (A) and IFN-γ (B) in the supernatants of cultured splenocytes stimulated with OVA (100 μg/ml). BALB/c mice were immunized s.c. with OVA (10 μg) alone in saline (200 pl) or OVA in saline (200 pl) containing alum (200 μg), Rg1 (50 μg), or alum (200 μg) plus Rg1 (50 μg) on days 1 and 21. Splenocytes were prepared and cultured with or without OVA (100 μg/ml) in RPMI 1640 after the mice were scarified 2 weeks after the last immunization. The culture supernatants were harvested after 72 h of incubation of the splenocytes with OVA. The values are presented as means ± standard deviations (n = 5). Values with different letters showed significant difference (P < 0.05).