FIG. 2.

Representative real-time RT-PCR amplification plot obtained with serial 10-fold dilutions (5 × 10¹ to 5 × 10⁷ copies per reaction) of HRV14 RNA transcript. The top panel shows a baseline subtractive curve fit (CF) view of the data, with relative fluorescence units (RFU) plotted against cycle numbers. The default setting of 10 times the standard deviation of the RFU measured in all wells over the baseline cycles was used to calculate the C_T for a positive reaction (horizontal line). The bottom panel shows a standard curve analysis of the DNA amplification plots with C_T values plotted proportionately against the logarithm of the input copy number. The dynamic range of assay spans 7 logs, with an R² value of 0.999.