TABLE 2.

Comparison of HRV real-time RT-PCR assay to reference assays for detection of HRV and HEV in clinical specimens^c

Virus detected	No. of samples	Results by the CDC HRV real-time RT-PCR		Results from laboratory:
				Aa				Bb
				HRV real-time RT-PCR		HEV real-time RT-PCR		Gel (HRV)		HEV real-time RT-PCR
		Pos	Neg	Pos	Neg	Pos	Neg	Pos	Neg	Pos	Neg
HRV	87	87	0	54	33	1	86	87	0	0	87
HEV	24	4	20	0	24	22	2	2	1	21	3

^aThe in-house assays used by laboratory A were hydrolysis probe real-time RT-PCR assays for HRV and HEV.

^bThe in-house assay used by laboratory B for HEV was a hydrolysis probe real-time RT-PCR assay. HEV-negative samples that subsequently yielded an appropriately sized band following gel electrophoresis were considered positive for HRV.

^cPos, positive; neg, negative.