TABLE 2.
DNA probes developed in this study
| Probe | Sequence (5′→3′) | % GCa | Tm (°C)a | Target species |
|---|---|---|---|---|
| U210 | TCG AGT TGT TTG GGA ATG CAG CTC | 50.0 | 65 | Panfungal |
| Ca170 | TGA GAT GAC CCG GGT CTG TGT AAA | 50.0 | 65 | Candida albicans |
| Ca176 | GAC CCG GGT CTG TGT AAA GTT CC | 56.5 | 66 | C. albicans |
| Cg175 | GTG TCA GTT CTT TGT AAA GGG TGC TCG | 48.0 | 68 | C. glabrata |
| Cd176 | GGC CCG GGT CTA TGT AAA GTT CC | 56.5 | 66 | C. dubliniensis |
| Cl180 | GAC TCT TTG CAC CGC GGC TCC | 66.7 | 67 | C. lusitaniae |
| Ct171 | GAG ATG ATC CAG GCC TAT GTA AAG T | 44.0 | 64 | C. tropicalis |
| Cp171 | GAG ATG TCC CAG ACC TAT GTA AAG TTC | 44.4 | 67 | C. parapsilosis |
| Ck175 | GGC GGA AGC AGT GAG GCC CTT CT | 65.2 | 70 | C. krusei |
| Pa176 | GCC CAT TCC TAT GTA AGG TGC TAT C | 48.0 | 66 | Pichia anomala |
| Sc176 | GTG CGG TTC TTT GTA AAG TGC CTT CG | 50.0 | 68 | Saccharomyces cerevisiae |
a
Data obtained with Oligo Analyzer, version 1.0.2 (Teemu Kuulasmaa, University of Kuopio, Finland).