TABLE 1.
Strains and clinical isolates used in this studya
| Species | Strain/isolate | Source | Location | recA RFLP result | Growth on BCSA | Amplification with JD490/JD491 | Amplification with species-specific primers |
|---|---|---|---|---|---|---|---|
| Burkholderia cepacia | CEP509*/LMG 18821* | CF | Australia | E | NT | + | + |
| ATCC 25416T*/LMG 1222T* | ENV | United States | D | NT | + | + | |
| ATCC 17759*/LMG 2161* | ENV | Trinidad | E | NT | + | + | |
| BCC group K | CEP0964 | CF | Canada | K | NT | + | + |
| CEP1056 | CF | Canada | K | NT | + | + | |
| R445 | CF | Canada | + | + | + | ||
| Burkholderia multivorans | LMG 13010T* | CF | Belgium | NT | + | + | |
| ATCC 17616*/LMG 17588* | ENV | United States | F | NT | + | + | |
| C3430 | CF | Canada | NT | + | + | ||
| C5274 | CF | Canada | NT | + | + | ||
| C5393*/LMG 18822* | CF | Canada | F | NT | + | + | |
| C5568 | CF | Canada | NT | + | + | ||
| M1512 | CF | Canada | + | + | + | ||
| M1865 | CF | Canada | + | + | + | ||
| R810 | CF | Canada | + | + | + | ||
| R1159 | CF | Canada | + | + | + | ||
| Burkholderia cenocepacia | K56-2*/LMG 18863* | CF-e | Canada | G | NT | + | + |
| J2315*/LMG 16656* | CF-e | United Kingdom | G | NT | + | + | |
| C1257 | CF-e | Canada | NT | + | + | ||
| C4455 | CF-e | Canada | NT | + | + | ||
| C5424*/LMG 18827* | CF-e | Canada | G | NT | + | + | |
| C6433*/LMG 18828* | CF-e | Canada | NT | + | + | ||
| CEP511*/LMG 18830* | CF-e | Australia | I | NT | + | + | |
| D1 | ENV | United States | NT | + | + | ||
| PC184*/LMG 18829* | CF-e | United States | J′ | NT | + | + | |
| LMG 19240 | ENV | Australia | NT | + | − | ||
| CEP0868/LMG 21461 | CF | Argentina | NT | + | + | ||
| R161 | CF | Canada | + | + | + | ||
| R452 | CF | Canada | + | + | + | ||
| R750 | CF | Canada | + | + | + | ||
| R1284 | CF | Canada | + | + | + | ||
| R1285 | CF | Canada | + | + | + | ||
| R1314 | CF | Canada | + | + | + | ||
| R1434 | CF | Canada | + | + | + | ||
| R1619 | CF | Canada | + | + | + | ||
| R1882 | CF | Canada | + | + | + | ||
| R1883 | CF | Canada | + | + | + | ||
| R1884 | CF | Canada | + | + | + | ||
| R2314 | CF | Canada | + | + | + | ||
| S11528 | CF | Canada | + | + | + | ||
| Burkholderia stabilis | LMG 14294T* | CF | Belgium | J | NT | + | + |
| C7322*/LMG 18870* | CF | Canada | NT | + | + | ||
| R450 | CF | Canada | + | + | + | ||
| R2140 | CF | Canada | + | + | + | ||
| R2339 | CF | Canada | + | + | + | ||
| Burkholderia vietnamiensis | LMG 10929T* | ENV | Vietnam | B | NT | + | + |
| PC259*/LMG 18835* | CF | United States | A | NT | + | + | |
| ATCC 29424 | ENV | United States | NT | + | + | ||
| G4 | ENV | United States | A | NT | + | + | |
| Burkholderia dolosa | AU0645T*/LMG 18943T* | CF | United States | Q | NT | + | + |
| CEP021*/LMG 21819* | CF | United States | NT | + | + | ||
| E12*/LMG 21820* | CF | United Kingdom | NT | + | + | ||
| L06 | CF | NT | + | + | |||
| STM1441*/LMG 21443* | ENV | Senegal | NT | + | + | ||
| Burkholderia ambifaria | ATCC 53266*/LMG 17828* | ENV | United States | L | NT | + | + |
| AMMDT*/LMG 19182T* | ENV | United States | N | NT | + | + | |
| CEP0996*/LMG 19467* | CF | Australia | N | NT | + | + | |
| M53 | ENV | United States | NT | + | + | ||
| Burkholderia anthina | W92T*/LMG 20980T* | ENV | United States | T | NT | + | + |
| C1765*/LMG 20983* | CF | United Kingdom | T | NT | + | + | |
| J2552*/LMG 16670* | ENV | United Kingdom | AS | NT | + | + | |
| AU1293*/LMG 21821* | CF | United States | AS | NT | + | + | |
| Burkholderia pyrrocinia | ATCC 15958T*/LMG 14191T* | ENV | Japan | NT | + | + | |
| ATCC 39277*/LMG 21822* | ENV | United States | P | NT | + | + | |
| BC011*/LMG 21823* | ENV | United States | AR | NT | + | + | |
| C1469*/LMG 21824* | CF | United Kingdom | AA | NT | + | + | |
| Burkholderia gladioli | R406 | CF | Canada | NA | + | + | NA |
| R1879 | CF | Canada | NA | + | + | NA | |
| Pseudomonas aeruginosa | R285 | CF | Canada | NA | − | − | NA |
| Herbaspirillum sp. | R740 | CF | Canada | NA | − | − | NA |
| Listeria monocytogenes | R1653 | CF | Canada | NA | − | − | NA |
| Pandoraea sp. | R1717 | CF | Canada | NA | + | − | NA |
| Burkholderia sp. | JS150 | ENV | United States | NA | NT | + | NA |
a
Abbreviations: RFLP, restriction fragment length polymorphism; CF, CF isolate; ENV, environmental isolate; CF-e, CF epidemic isolate; NA, not applicable; NT, not tested. Strains in boldface type were used to test each of the nine species-specific primer sets. Asterisks indicate strains of the BCC experimental strain panel (10, 22).