TABLE 2.
Species-specific primers used for amplification of the BCC fur gene
| Species | Forward primer | Forward primer sequence | Forward primer positions | Reverse primer | Reverse primer sequence | Reverse primer positions | Product length (bp) | BCC species amplified |
|---|---|---|---|---|---|---|---|---|
| B. cepacia/BCC group K | F1 | GGCNGAAGACGTCTACCGG | 102-120 | R1 | TCGAAGTTGCTGCGCGAC | 201-218 | 117 | B. cepacia |
| B. multivorans | F2 | AGCAGAGCCCCGTGCGG | 77-93 | R2 | GGTGGGGGCAGTTTTCGGTG | 399-418 | 342 | B. multivorans |
| B. cenocepacia | F | TGACCAATCCGACCGATCTCA | 2-22 | R3 | ATCGCCTGCTGGCGGCTC | 321-338 | 337 | B. cenocepacia IIIA, IIIB, IIID |
| B. stabilis | F4 | CNACCGTCTATCGCGTGCTC | 155-174 | R | TCAGTGCTTGCGGTGGGG | 412-429 | 275 | B. multivorans, B. cenocepacia, B. stabilis, B. dolosa |
| B. vietnamiensis | F | TGACCAATCCGACCGATCTCA | 2-22 | R5 | CGTGGTGGGAGCCTTCGTTG | 243-262 | 261 | B. vietnamiensis |
| B. dolosa | F6 | CTAAAGGCCACCCTACCGCGG | 34-54 | R | TCAGTGCTTGCGGTGGGG | 412-429 | 396 | B. dolosa |
| B. ambifaria | F7 | CCCNGTGCGTCACCTGACT | 84-102 | R7 | CGTGGTGCGAACCTTCATTCAA | 241-262 | 179 | B. ambifaria |
| B. anthina | F | TGACCAATCCGACCGATCTCA | 2-22 | R8 | CAGGTGACGCACGGGGCTC | 81-99 | 98 | B. multivorans, B. cenocepacia, B. stabilis, B. anthina, B. pyrrocinia (1 strain) |
| B. pyrrocinia | F1 | GGCNGAAGACGTCTACCGG | 102-120 | R9 | ATCGCCTGCTGGCGGCC | 322-338 | 237 | B. pyrrocinia |
| JD490 | ATGACCAATCCGACCGATCTCAA | 1-23 | JD491 | TCAGTGCTTGCGITNIGGGCAGTT | 406-429 | 429 | All |