TABLE 2.
Biochemical properties of C. freneyi and C. xerosis
| Category | % Positive reactionsa
|
|
|---|---|---|
| C. freneyi (n = 21) | C. xerosis (n = 2) | |
| Nitrate reduction | 67 | 50 |
| Urea hydrolysis | 0 | 0 |
| Esculin hydrolysis | 0 | 0 |
| Pyrazinamidase | 100 | 100 |
| Pyrrolidonyl arylamidase | 0 | 0 |
| Gelatinase | 0 | 0 |
| CAMP | 0 | 0 |
| Lipophilia | 0 | 0 |
| Fermentation | ||
| Glucose | 100 | 100 |
| Maltose | 100 | 100 |
| Sucrose | 100 | 100 |
| Mannitol | 10 | 0 |
| Xylose | 0 | 0 |
| Ribose | (100) | 100 |
| Lactose | 48 | 0 |
| Glycogen | (48) | 0 |
| Glycerol | 0 | 50 |
| Erythritol | 0 | 0 |
| d-Arabinose | 0 | 0 |
| l-Arabinose | (5) | 0 |
| Adonitol | (10) | (50) |
| β-Methyl-xyloside | (5) | 0 |
| Galactose | 95 | 50 |
| d-Fructose | 100 | 100 |
| d-Mannose | 100 | 100 |
| l-Sorbose | 10 | 0 |
| Rhamnose | 10 | 0 |
| Dulcitol | (10) | 50 |
| Inositol | (19) | 50 |
| Sorbitol | (14) | 50 |
| α-Methyl-d-mannoside | (10) | 0 |
| α-Methyl-d-glucoside | (10) | 0 |
| N-Acetyl-glucosamine | (10) | 0 |
| Amygdaline | (10) | 0 |
| Arbutine | (24) | 0 |
| Salicine | (10) | 50 |
| Cellobiose | (19) | 0 |
| Melibiose | (10) | 0 |
| Trehalose | 100 | 100 |
| Inuline | (14) | 0 |
| Melezitose | (19) | 0 |
| d-Raffinose | (10) | 0 |
| Amidon | (5) | 0 |
| Xylitol | (29) | 0 |
| β-Gentibiose | (33) | 50 |
| d-Turanose | 100 | 100 |
| d-Lyxose | (14) | 50 |
| d-Tagatose | (100) | (50) |
| d-Fucose | (29) | 50 |
| l-Fucose | (19) | 0 |
| d-Arabitol | (24) | 0 |
| l-Arabitol | (19) | 0 |
| Gluconate | (43) | 0 |
| 2-Keto-Gluconate | 0 | 0 |
| 5-Keto-Gluconate | 100 | 100 |
| Further enzymatic activitiesb | ||
| Alkaline phosphatase | 100 (m) | 100 (w) |
| Esterase (C4) | 100 (m) | 100 (m) |
| Esterase lipase (C8) | 100 (s) | 100 (s) |
| Lipase (C14) | 14 (w) | 50 (w) |
| Leucine arylamidase | 95 (s) | 100 (s) |
| Valine arylamidase | 0 | 0 |
| Cystine arylamidase | 0 | 100 (w) |
| Trypsin | 0 | 0 |
| Chymotrypsin | 0 | 0 |
| Acid phosphatase | 19 (w) | 100 (w) |
| Phosphoamidase | 100 (m) | 100 (w) |
| α-Galactosidase | 0 | 0 |
| β-Galactosidase | ||
| pH 5.4 | 0 | 0 |
| pH 7.4c | 0 | 0 |
| β-Glucuronidase | 0 | 0 |
| α-Glucosidase | ||
| pH 5.4 | 100 (s) | 100 (s) |
| pH 7.4c | 100 (s) | 100 (s) |
| β-Glucosidase | 0 | 0 |
| N-Acetyl-β-glucosaminidase | 0 | 0 |
| α-Mannosidase | 0 | 0 |
| α-Fucosidase | 0 | 0 |
a
Values in parentheses represent weak acid production after 120 h incubation. (w), hydrolyzed substrate (approximately 5 nmol); (m), hydrolyzed substrate (approximately 20 nmol); (s), hydrolyzed substrate (>40 nmol).
b
As determined by use of the API ZYM system.
c
As determined using the API Coryne system.