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. 1999 Mar 30;96(7):3836–3841. doi: 10.1073/pnas.96.7.3836

Figure 2.

Figure 2

Xist expression in Tg ES and EB cells. (A) RNA FISH of undifferentiated ES cells with a rhodamine-labeled Xist probe (red). X, endogenous; T, Tg; Blue, 4′,6-diamidino-2-phenylindole (dapi) staining. (B) RNA FISH of day 9 EB cells (Xist RNA, red). Double Xist expressers are indicated by asterisks; the arrow indicates Xist RNA coat on a πJL2.5 metaphase chromosome. (C) RNA/DNA FISH for Xist RNA (red) and Zfx or vector DNA (FITC, green) was performed for all ES lines to determine Xist RNA origin. πJL2.5 EB is shown. Coincident red and green resulted in white.