Table 2.
Pattern of Xist expression in Tg EB cells
| ES line | Double expressors, %
|
Single expressors, %
|
Xist-expressing cells in day 9 EB cultures, % | |
|---|---|---|---|---|
| Endogenous + Tg | Endogenous only | Tg only | ||
| F7.26 | 65 | 0 | 35 | 100 |
| F9.18 | 60 | 0 | 40 | 100 |
| R3.6 | 50 | 0 | 50 | 65 |
| R9.11 | 75 | 0 | 25 | 100 |
| F21.21 | 60 | 0 | 40 | 80 |
| F47.2 | 55 | 0 | 45 | 75 |
| R42.19 | 80 | 0 | 20 | 100 |
| πJL1.4 | 35 | 0 | 65 | 90 |
| πJL2.4 | 50 | 0 | 50 | 80 |
| πJL3.10 | 20 | 0 | 80 | 30 |
| 116.6 | 45 | 0 | 55 | 90 |
| N1.1 | — | — | — | 10 |
| J1 | — | — | — | <10 |
The right column indicates the percentage of Xist-expressing cells in the entire day 9 EB culture. Numbers in column 2, 3, and 4 are the percentages of double or single expressers of all Xist-expressing cells. Percentages are rounded off to nearest fifth percentile and were gathered in three experiments (n > 200 nuclei for each experiment).