TABLE 2.

Primers used in the MALDI-TOF MS analyses

Primer (NCBI reference sequence accession no., target gene)	Sequence (5′-3′)a	Primer 5′-position	UEPc size (Da)	Extension base(s)	EPd size (Da)
HSV1 (NC_001806, UL5)	F: CGTGGAGCTTGGCCATGTAC	13904
	R: AACCTCATGAAGGTGCTGGAGTAC	14075
	ME: GGAGCACATGCAGTT	14034	4,617	C	4,906
HSV2 (NC_001798, UL5)	F: CGTGGAGCTTGGCCATGTAC	14025
	R: AACCTCATGAAGGTGCTGGAGTAC	14196
	ME: GGAGCACATGCAGTT	14155	4,617	T	4,921
CMVb (X92746, UL122)	F: GCCAAGCGGCCTCTGATAAC	484
	R: TCATCCACACTAGGAGAGCAGACT	620
	ME: ATGCAGATCTCCTCAA	554	4,825	T	5,129
VZV (AY548171, ORF1)	F: CAACGTCGCCATCTGGAGTAC	652
	R: GCCACTCGTCGTTCAAACAAG	805
	ME: AGGTTATGCAACGTACT	696	5,209	GGGT	6,501
EBV A (K03333, EBNA2)	F: ACCTGATTCCCCCTGCTCAT	716
	R: CATTGGAAGGTGGCTGGTGAT	868
	ME: GTAGTGGCTTGGTTGT	761	4,974	A	5,287
EBV B (K03332, EBNA2)	F: ACCTGATTCCCCCTGCTCAT	713
	R: CATTGGAAGGTGGCTGGTGAT	865
	ME: GTAGTGGCTTGGTTGT	758	4,974	GA	5,616
HHV8 (U93872, ORF06)	F: AAAAAGAATAATGTCAGCTCTATGCTAAGA	6074
	R: TTAACCGCTTCTGCCTCGAA	6195
	ME: GATTATGACCCCCCT	6115	4,488	GGT	5,451
HHV6A (X83413, U66)	F: ATGAACCACGTTTTTCCATGTCTTCT	101834
	R: GAACCATTTCAGAAGAACATTCTCATAC	102033
	ME: CTAACGTATTCCTAGTCCC	101879	5,699	A	6,012
HHV6B (AF157706, U66)	F: ATGAACCACGTTTTTCCATGTCTTCT	103132
	R: GAACCATTTCAGAAGAACATTCTCATAC	103331
	ME: CTAACGTATTCCTAGTCCC	103177	5,699	GA	6,341
HHV7 (AF037218, U60)	F: ATGAACCATGTTTTACCGTGTCTTCT	99572
	R: GAGCCATTTCAAAAGAGCATTCTTATAC	99771
	ME: AGCTTTCTGTTTAAGTATG	99616	5,824	T	6,128

^aThe 5′ extension ACGTTGGATG was added to all forward and reverse primers. F, forward; R, reverse; ME, MassExtend.

^bThe forward and reverse primers were adapted from those of Mengoli et al. (18).

^cUEP, unextended primer.

^dEP, extended primer.